FIGURE 1.

Correlation between SLUG and cyclin D1 levels in breast tissues and cells. A, human breast cancer tissue microarray was examined for cyclin D1 and SLUG expressions by immunohistochemistry using mouse anti-cyclin D1 and rabbit anti-SLUG antibodies. A low magnification (×4) immunofluorescence micrograph of the array is shown in the supplemental material (supplemental Fig. 1S). We show here a higher magnification (×30) immunofluorescence micrograph for selected spots. The coordinates of the spots are marked as detailed in supplemental Table 3S. B, evaluation of the levels of cyclin D1 in normal and SLUG-expressing human breast cancer cells by immunofluorescence microscopy. 468C and MCF7C, MDA-MB-468 and MCF7 cells transfected with empty vector; 468SLUG and MCF7SLUG, cells expressing C-terminal FLAG-tagged SLUG; 231C and BT549C, normal MDA-MB-231 and BT549 cells.