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. 2010 Nov 2;286(1):469–479. doi: 10.1074/jbc.M110.164384

FIGURE 2.

FIGURE 2.

Effect of SLUG overexpression on the levels of cyclin D1 in MDA-MB-468 cells. A, increase in cyclin D1 levels in SLUG-expressing MDA-MB-468 cells. A Western blot shows higher levels of cyclin D1 in the recombinant cells. B, densitometric scan for cyclin D1 and SLUG levels in six independent SLUG-transfected populations and vector controls. Results are mean ± S.E. (error bars) (n = 6). The -fold changes were statistically significant (p < 0.001). C, evaluation of SLUG, cyclin D1, and UbcH5c protein levels in four different breast cancer cell lines by Western blotting. Bands were developed using IR dye-conjugated secondary antibody (LI-COR Biosciences) and visualized using the LI-COR Odyssey infrared imaging system. Quantitation and analysis of bands were performed using Odyssey software. β-Actin was used as normalization control. Levels of SLUG and cyclin D1 in the MDA-MB-231 cells and that of UbcH5c in the MCF7 cells were taken as 100 for comparison. Results are mean ± S.E. (n = 4).