FIGURE 6.

Effect of overexpression of UbcH5c on cyclin D1 levels and proliferation in SLUG-high MDA-MB-231 cells. A, immunoblot analysis showing the effect of overexpression of UbcH5c on cyclin D1 level. Control cells were transfected with empty vector DNA. UbcH5c levels were assessed by using Myc antibody for the recombinant protein and UbcH5c antibody for the total of the endogenous and recombinant protein. B, proliferation assays with the control and UbcH5c-overexpressed (UbcH5cOE) MDA-MB-231 cells. Results are mean ± S.E. (error bars) (n = 6).