FIGURE 7.

Inhibition of the UbcH5c promoter activity in SLUG-expressing human breast cells. A, ChIP analysis for the binding of SLUG to the promoter of the UbcH5c gene in FLAG-tagged SLUG-expressing (+SLUG) MCF7 and MDA-MB-468 cells. Immunoprecipitation (IP) was done with FLAG antibody to immunoprecipitate chromatin fragments bound to FLAG-tagged SLUG. B, ChIP analysis for the binding of SLUG to the promoter of the UbcH5c gene in the control and the SLUG-knocked down MDA-MB-231 and BT549 (−SLUGKD) cells. ChIP grade SLUG antibody was used for immunoprecipitation of wild-type SLUG-bound chromatin fragments. C, repression of the UbcH5c promoter in the SLUG-expressing (SLUGOVEX) MCF7 and MDA-MB-468 cells. D, effect of E2-box mutation at the UbcH5c promoter on its activity in SLUG-expressing MCF7 cells. In C and D, the averages from six different SLUG-transfected populations and controls are shown. Results are mean ± S.E. (error bars) (n = 6). Decreases in the luciferase activities were statistically significant (p < 0.001).