FIGURE 8.

Mechanism of repression of UbcH5c promoter by SLUG in breast cancer cells. A, ChIP analysis for the co-recruitments of CtBP1 and HDAC1 with SLUG at the UbcH5c promoter. B, immunoblot analysis for the effect of trichostatin A (TSA) on SLUG-induced repression of UbcH5c levels in SLUG-expressing MDA-MB-468 cells. C, ChIP analysis showing the decrease of acetylated histones H3 and H4 at the UbcH5c promoter in SLUG-expressing MDA-MB-468 cells. D, quantitative ChIP analysis to evaluate the levels of acetylated histones H3 and H4 at the UbcH5c promoter in SLUG-expressing MDA-MB-468 cells. *, statistical significance (p < 0.001). E, model for the regulation of UbcH5c promoter by SLUG in human breast cancer cells by chromatin remodeling. BD, DNA binding domain of SLUG; RD, repressor domain for SLUG. Error bars, S.E.