FIGURE 3.

Inhibition of TM-dependent TAFI activation by PF4 and loss of TAFIa-mediated BK inactivation in normal plasma. A, time course of BK (closed symbols) conversion to des-Arg⁹-BK (BK-dR⁹, open symbols) during tissue factor-induced coagulation in normal pooled plasma (▴ and △, no TM and no PF4; ♦ and ♢, 50 μg/ml PF4; □ and ■, 0.3 nm TM; and ○ and ●, 0.3 nm TM + 50 μg/ml PF4). B, PF4 effect on BK (closed symbols) conversion to des-Arg⁹-BK (open symbols) during tissue factor-induced coagulation in normal pooled plasma (□ and ■, no TM; and ○ and ●, 0.3 nm TM). C, TM effect on BK (closed symbols) conversion to des-Arg⁹-BK (open symbols) during tissue factor-induced coagulation in normal pooled plasma (□ and ■, no PF4; ○ and ●, 50 μg/ml PF4; and ♦ and ♢, 100 μg/ml PF4). Each point represents the mean ± S.E. from at least three independent experiments.