FIGURE 6.

Reversal of PF4-induced inhibition of TAFIa-mediated clot protection in hemophilia A plasma on TM-bearing cells by non-anticoagulant heparin derivatives. A, clot lysis initiated in factor VIII-deficient plasma by thrombin (10 nm) and tPA on TM-expressing K293 cells in the absence (♦) and presence (♢) of CPI 20 μg/ml. A representative experiment is shown. B, clot lysis initiated in factor VIII-deficient plasma by thrombin (10 nm) and tPA (60 units/ml) on TM-expressing K293 cells in the absence (light bars) and presence (dark bars) of 50 μg/ml PF4. Blocking antibodies against TM (20 μg/ml) preincubated with cells 10 min before the start of the experiment, CPI (20 μg/ml) and NAc-Hep were included as indicated. Each point represents the mean ± S.E. from at least three independent experiments.