TABLE 2.
Peptide binding affinity of SETDB1 peptides
Dissociation constant values (Kd) for Cbx7 and Cbx8 chromodomains with consensus SETDB1-methylated peptides. Kd values represent the averages ± S.E. for at least two independent experiments. N/B, no binding.
| Motif | SETDB1 consensus sequence | Cbx7 Kd | Cbx8 Kd |
|---|---|---|---|
| μm | μm | ||
| VAVK(1162)S | RQVAVKme3STR | 43 ± 4 | 98 ± 15 |
| FALK(1170)S | 1165-RGFALKme3STHG | 5 ± 0.5 | 29 ± 6 |
| IAIK(1178)S | HGIAIKme3STN | 5 ± 0.8 | 37 ± 4 |
| Kme3(1162,1170,1178) | RQVAVKme3STRGFALKme3STHGIAIKme3STN | 1.2 ± 0.2 | 6 ± 2 |
| Kme2(1162,1170,1178) | RQVAVKme2STRGFALKme2STHGIAIKme2STN | 15 ± 0.5 | 180 ± 80 |
| Non-methylated | RQVAVKSTRGFALKSTHGIAIKSTN | N/B | N/B |