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Identification of the AP-1 and CREB/ATF proteins that bind to region I (−54 to −38) of the hNT/N promoter. Nuclear extracts (10 μg/lane) from BON cells were preincubated with antisera (2 μl) to various AP-1 and CREB/ATF proteins prior to the addition of labeled WT region I probe. Supershifted complexes were noted for c-Jun (lane 4), JunD (lane 5), ATF-1 (lane 11), ATF-2 (lane 12), and CREB (lane 13).
