FIGURE 1.

S. cerevisiae COX25 codes for a protein required for respiratory growth. A, sequence alignment of COX25 from S. cerevisiae (Sc) and other fungi: Neurospora crassa (Nc), Aspergillus nidulans (An), Podospora anserina (Pa), Gibberella zeae (Gz), Kluiveromyces lactis (Kl), Pichia pastoris (Pp), and Yarrowia lipolytica (Yl). TM indicates a predicted transmembrane domain. B, the respiratory competent wild-type strains BY4741 and W303 and strains carrying a null allele of cox25 in both genetic backgrounds were grown overnight in liquid YPD media. 10-fold serial dilutions of the four strains were plated on solid YPD or YPEG media and incubated at 30 °C. The pictures were taken after 3 days of incubation. C, wild-type W303 and mutant Δcox25 cells overexpressing or not COX25 were grown overnight in liquid YPD media. Serial dilution growth test was performed as for B.