FIGURE 6.

Determination of the size and relative expression levels of dupα7 mRNA in the monosomal and polysomal fractions of HL-60 cells. A, Northern blot performed with RNAs from human CTX and HL-60 cells showing the 3.9-kb transcript in both lanes and different α7 transcript of 5.8, 3.3, 2.1, and 1.4 kb in CTX. B, levels of dupα7 or TBP mRNAs in monosomal and polysomal fractions of HL-60 cells quantified by Q-PCR. The results are expressed as a percentage of each messenger in the two fractions. Values were obtained in triplicate and correspond to two different cultures of HL-60 cells subjected to ultracentrifugation on a sucrose gradient as described under “Experimental Procedures.”