FIGURE 8.

Role of Cbl in Vav2 ubiquitinylation. 16A5-Tet-On-172F cells with stable expression of vector, wild type Cbl (A and B), Cbl-Y700F, or Cbl-C3AHN (A) or stable expression of control shRNA or Cbl-shRNA#4 (B) were treated with DOX for 3 days to induce the expression of Vav2-Y172F. The cells were then treated with DMSO or MG132 (100 μm) for 4 h before lysis. Cell lysates were subjected to IP with anti-Vav2 Abs and immunoblotted for ubiquitin (Ub) and Vav2. Whole cell lysates were also blotted for Cbl.