FIGURE 2.

Identification and characterization of exosome-like vesicles secreted from primary cultures of oligodendrocytes. A, the medium of primary oliogodendrocytes cultured for 6–8 days was collected and submitted to sequential centrifugation steps (3000 × g pellet (P3); 4000 × g pellet (P4); 10,000 × g (P10), and 100,000 × g (P100)) as indicated. The resulting pellets of each centrifugation step were analyzed by Western blotting for PLP. B, cell lysates (CL) and 100,000 × g pellets (P100) were analyzed by Western blotting for the indicated proteins. C, the 100,000 × g pellet was prepared and negatively stained with 1% uranyl acetate. Scale bar, 50 nm.