Skip to main content
. 2010 Oct 18;286(1):79–90. doi: 10.1074/jbc.M110.169821

FIGURE 5.

FIGURE 5.

ErbB2 regulates EGFR and β1 integrin via Erk-dependent regulation of Sprouty2. A, MCF-10A or MCF-10A ErbB2 cells treated with 20 μm PD98059(+) or vehicle (−) were plated under attached or detached conditions for 24 h. mRNA levels were measured by qPCR. Column A, ECM-attached; column D, ECM-detached. B, MCF-10A, MCF-10A ErbB2, or MCF-10A MekDD cells were plated under attached or detached conditions for 24 h, and mRNA levels were measured by qPCR. C, MCF-10A cells infected with pMSCV-IRES-PURO or pMSCV-SPRY2-IRES-PURO were plated under attached or detached conditions for 24 h, and protein levels were analyzed by immunoblotting. D, MCF-10A or MCF-10A ErbB2 cells infected with pMSCV-SPRY2-IRES-PURO were plated under attached or detached conditions with 10 μm UO126 or 20 μm PD98059 (+) or vehicle (−). 24 h later, protein levels were analyzed by immunoblotting. pErk1/2, phosphorylated Erk1/2. E, cells were plated and analyzed as described in C. F, MCF-10A ErbB2 cells were infected with shRNA vectors targeting Sprouty2, and mRNA levels were measured by qPCR to confirm knockdown. G and H, MCF-10A ErbB2 cells infected with shRNAs targeting Sprouty2 were plated under detached (G) or attached (H) conditions for 24 h, and protein levels were measured by immunoblotting. I, SK-BR-3 cells were infected with shRNA vectors targeting Sprouty2, and mRNA levels were measured by qPCR to confirm knockdown. J and K, SK-BR-3 cells infected with shRNA vectors targeting Sprouty2 were plated under detached (J) or attached (K) conditions for 24 h, and protein levels were measured by immunoblotting.