FIGURE 5.

Gephyrin co-localizes with GlyRs in the spinal cord. A, protein extracts from whole zebrafish embryos (48 hpf) were separated by SDS-PAGE and probed with an anti-gephyrin antibody, resulting in the detection of a major ∼93-kDa protein. B–F, immunostaining of zebrafish spinal cord sections (48 hpf). Cross-sections were co-labeled with anti-gephyrin and anti-Islet1 (B), with anti-GlyRα and anti-Islet1 (C), with anti-GlyRα and anti-gephyrin (D), with anti-GluR and anti-gephyrin (E), or with anti-synaptic vesicles and anti-gephyrin (F). Islet1-positive cells are motor neurons (ventral side) and mechanosensory Rohon-Beard neurons (dorsal side). Note that gephyrin is co-localized with GlyRs but not with GluRs.