FIGURE 6.

Inhibition of zebrafish gephyrin translation. Control, gphna, or gphnb MO or a mixture of gphna/gphnb MOs were injected into wild-type embryos. Cross-sections of these morphants (48 hpf) were immunolabeled with anti-gephyrin (A–H) or anti-GlyR (I–P). Expression of gephyrin in the lateral spinal cord was observed in control, gphna, and gphnb morphants (A–C) but not in gphna/gphnb double morphants (D). High magnification images of the lateral spinal cord revealed that gephyrin immunoreactivities are puncta in control, gphna, and gphnb morphants (E–G). Gephyrin protein was eliminated by knocking down both gphna and gphnb (H). GlyR clusters were observed in control, gphna, and gphnb morphants (I–K) but not in gphna/gphnb double morphants (L). High magnification images (M–P) confirm that unlike control or gphna and gphnb morphants (M–O), GlyR immunoreactivity in gphna/gphnb double morphants is weak and diffuse, presumably representing GlyRs trapped in intracellular compartments (P).