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Ligand-induced activation of transcription factors. A, ligand-induced AP-1- and CRE-mediated transcription in WT and TamR cells. AP-1 and CRE transcriptional activity was measured by a luciferase reporter assay. The mean of triplicate experiments is shown. The error bar represents standard deviation. B, Western blot analysis of transcription factors. E, E2; H, HRG. The phosphorylated and total protein levels were detected with specific antibodies.
