Table 1. List of PCR primers and the primary antibodies.
| Gene | Oligonucleotides | PCR product size | Primary antibodies |
|---|---|---|---|
| Il1b | F: GACCTTCCAGGATGAGGACA | 283 bp | PR-427β (Endogen) |
| R: AGGCCACAGGTATTTTGTCG | |||
| Il6 | F: ATGGATGCTACCAAACTGGAT | 138 bp | AMC0864 (Biosource) |
| R: TGAAGGACTCTGGCTTTGTCT | |||
| Il10 | F: GGTTGCCAAGCCTTATCGGA | 190 bp | |
| ACCTGCTCCACTGCCTTGCT | |||
| Tgfb1 | F: TGAGTGGCTGTCTTTTGACG | 292 bp | |
| R: TCTCTGTGGAGCTGAAGCAA | |||
| Ccl2 | F: AGGTCCCTGTCATGCTTCTG | 279 bp | Sc-1784 (Santa Cruz) |
| R: ATTTGGTTCCGATCCAGGTT | |||
| Ccl5 | F: AGCAGCAAGTGCTCCAATCT | 280 bp | |
| R: ATTTCTTGGGTTTGCTGTGC | |||
| Cxcl10 | F: GCTGCAACTGCATCCATATC | 293 bp | Sc-1406 (Santa Cruz) |
| R: CACTGGGTAAAGGGGAGTGA | |||
| Icam1 | F: TGGTGATGCTCAGGTATCCA | 273 bp | |
| R: CACACTCTCCGGAAACGAAT | |||
| Vcam1 | F: GTGGTGCTGTGACAATGACC | 287 bp | |
| R: ACGTCAGAACAACCGAATCC | |||
| Cybb | F: GACTGCGGAGAGTTTGGAAG | 277 bp | |
| R: ACTGTCCCACCTCCATCTTG | |||
| Nos2 | F: CAGAGGACCCAGAGACAAGC | 299 bp | |
| R: TGCTGAAACATTTCCTGTGC | |||
| Actb | F: CACCCTGTGCTGCTCACC | 327 bp | |
| R: GCACGATTTCCCTCTCAG |