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. 2010 Dec 31;5(12):e14481. doi: 10.1371/journal.pone.0014481

Figure 1. RPB3 interacts with eEF1γ.

Figure 1

A: Yeast two hybrid assay: AH109 yeast cells were co-transformed with the indicated constructs and plated onto SD media lacking leucine and tryptophan (-LW) to verify the expression of both bait (W+) and prey (L+) plasmids, or onto media lacking leucine, tryptophan, histidine and adenine (-LWHA) to examine the interaction between bait and prey proteins. B: Schematic representation of full-length proteins of both bait (RPB3) and prey (eEF1γ). C: Whole cell extracts of HeLa cells transfected with either myc-eEF1γ or flag-RPB3 and myc-eEF1γ were immunoprecipitated with the anti-flag monoclonal antibody and the co-immunoprecipitation was analysed by western blot using the anti-myc monoclonal antibody. The myc-eEF1γ signal is above the heavy chain Ig band. D: Whole cell extracts of HeLa cells transfected with either myc-eEF1γ or with myc-eEF1γ and EGFP-RPB3 were immunoprecipitated with the anti-myc monoclonal antibody and the co-immunoprecipitation was analysed by western blot using the anti-EGFP monoclonal antibody. The EGFP-RPB3 signal is above the heavy chain Ig band.