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. 2001 Feb 13;98(5):2289–2294. doi: 10.1073/pnas.041609798

Figure 1.

Figure 1

The sequence of C. reinhardtii rbcL from +1 (the transcription start site) through +350. Translation starts at +93. The basic promoter of this gene extends to +63 (2) and a transcriptional enhancer sequence lies between +63 and +170 (2). In some of the present experiments reporter genes were tested that contained the first 10 (+1 to +10), 20 (+1 to +20), 41 (+1 to +41), or 62 (+1 to +62) nucleotides of rbcL introduced downstream of the C. reinhardtii chloroplast atpB promoter (Figs. 3 and 4). In other experiments (Figs. 6 and 7), reporter genes were tested that contained the −709 to +350 segment of rbcL followed by a uidA (GUS) or aadA protein coding sequence and the 3′ terminal sequence of the psaB gene. Except for the control reporter, each reporter gene had a mutation at ST27, ST3, ST4, ST5, ST6, ST7, ST9, ST11, ST12, ST14, ST15, or ST30. A reporter gene with mutations at both ST27 and ST30 was also tested. Each mutagenized region is over- or underlined, and the nucleotides substituted within each mutated region are shown.