Figure 3. Influence of IL-2 on CD4⁺ T cell apoptosis.

A. Apoptosis of CD4⁺CD25⁻ (n = 5) and CD4⁺CD25⁺ T cells (n = 4) isolated from new onset diabetic NOD females and incubated for 48 hours with and without 2000 U/ml IL-2. B. Demonstrative measurements of proliferation rates of isolated CD25⁻ and CD25⁺ cells as determined from CFSE dilution (representative of 4 independent measurements). C. Impact of exogenous IL-2 supplementation on apoptosis measured by gating on CD25⁻ and CD25⁺ subsets in mixed cultures (n = 4). D. Demonstrative plots of CFSE dilution in gated CD4⁺ subsets within mixed cultures (representative of 4 measurements).