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. 2010 Dec 31;5(12):e15684. doi: 10.1371/journal.pone.0015684

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Kaminitz et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Representative plots demonstrating upregulation of CD25 in response to CD3/CD28 stimulation in mixed splenocyte cultures. B. Spontaneous apoptosis in isolated (n = 4) and gated cells (n = 5) in reference to responsiveness (CD25 expression) to CD3-activation, assuming negligible contribution of naturally occurring CD25⁺ T cells in mixed cultures. C. Proliferation rates under CD3-activation of responsive (CD25⁻→CD25⁺) and non-responsive (CD25⁻) subsets in isolated CD25⁻ T cells (n = 4) and in mixed cultures (gated, n = 5). D. Fractional apoptosis of responsive and non-responsive cells to CD3/CD28 stimulation in mixed cultures (n = 5). E. Proliferation rates under CD3/CD28 stimulation.