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. 2010 Dec 31;5(12):e15907. doi: 10.1371/journal.pone.0015907

Figure 5. Co-localization of Pax2 and Sox2 with GFP driven by the HCNR 81675 enhancer.

Figure 5

(A–D) Dorsal view of transgenic embryos assayed by ISH for the expression of pax2a (A), sox2 (B), sox10 (C) at 13, 15 and 18 hpf. GFP (D) displays a similar pattern than sox2 and pax2a at 15 hpf (compare A and B with D). Orientation is anterior to the left. (E–E″) Double immunostaining with anti-Pax2 (E) and anti-GFP antibody (E′) in transverse sections of 15 hpf otic vesicles revealed co-localization of both proteins (E″). (F–F″) GFP protein (F′) also co-localizes with sox2 mRNA (F″) but not sox10 or tbx1 mRNA (G″ and H″). (I–P) pax2a and sox2 expression is abolished in retinoic acid treated HCNR 81675 embryos (compare J and L to I and K, respectively) but not other genes such as sox10 or neuroD (compare N and P to M and O, respectively). Dorsal view, orientation is anterior to the left.