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Specificity of the mutant protein band confirmed by gene-specific knockdown in transfectants of siRNA targeting EP300. (A) qRT-PCR data to assess siRNAs targeting EP300 (best knockdown using P300-1 and P300-3). (B–C) Decreased mutant EP300 protein detected in Western Blot analyses on immunoprecipitates of EP300 siRNA transfectants relative to untransfected controls (PBS).
