Table 2. Representative cMS mutation analysis in colon cancer cell lines.

Gene	BAX	MSH3	TGFBR2	CASP5	MBD4	ACVR2A	ACVR2A	CREBBP	EP300	AIM2	TTK	PTHLH	TCF7L2
cMS	G8	A8	A10	A10	A10	A8 (1st)	A8 (2nd)	C5	A5	A10	A9	A11	A9
Cell line	NMD-S	NMD-S	NMD-S	NMD-S	NMD-S	NMD-S	NMD-R	NMD-R	NMD-R	NMD-R	NMD-R	NMD-R	NMD-R
SW1222	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	-	wt
SW480	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt
LIM1863	wt	wt	wt	wt	wt	wt	−1	wt	wt	wt	wt	wt	wt
LIM2099	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt
LIM2463	wt	wt	wt	wt	wt	wt	−1, wt	wt	wt	wt	wt	wt	wt
HT29	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt	wt
HCA-7	−1	−1	−1	+1, wt	−1	wt	−1	wt	wt	−1, wt	wt	−2, wt	+1, wt
HCT116	−1	−1	−1	−1, wt	−1, wt	wt	−1	wt	−1, wt	−1, wt	−1, wt	−2	wt
LoVo	−1, +1	wt	−2, −1	−1, wt	wt	wt	−1	−1, wt	wt	−1	−1, wt	−1	−1, wt
LS174T	−1	wt	−2, −1	−1	wt	wt	−1	wt	wt	wt	−1, wt	−1, wt	−1, wt
LIM1215	−1	−1, wt	−1	−1	wt	wt	−1, wt	wt	wt	−1, wt	−1, wt	−1	wt
LIM1899	−1	wt	−2, +1	−1, wt	−1, wt	wt	−1	wt	wt	wt	−1, wt	−1, wt	wt
LIM2405	wt	−1, wt	wt	−2, −1	wt	−1	−1, wt	wt	wt	wt	wt	−1, wt	wt
LIM2408	wt	−1, wt	wt	−2, −1	wt	−1	−1, wt	wt	wt	−1, wt	wt	−1, wt	−1, wt
LIM2537	−1, wt	wt	−2, −1	−2, wt	−1, wt	wt	−1	wt	wt	−2, −1	−1, wt	−1, wt	wt
LIM2550	wt	wt	−1, wt	−1, wt	−1, wt	wt	−1	wt	wt	−2, −1	−1, wt	−1, wt	wt
LIM2551	wt	wt	−1	−2, wt	wt	wt	−1, wt	wt	wt	−1, wt	−1, wt	−1, wt	−1, wt

−1 = deletion of 1 base in cMS; wt = wild type. NMD-S: predicted NMD-sensitive mutant transcript; NMD-R: predicted NMD-resistant mutant transcript. ACVR2A: A8 (1^st) and A8 (2^nd) refer to two separate cMS in order of coding sequence.