Figure 1.

(A) DNA sequence of the 3′ fragment (from +723 to +983) of the maize rbcS-m3 gene. The regions that were mutated, one region in each DNA tested in the in situ transient expression assay, are marked and named according to the designation of the primers used to introduce the mutation. The substituted bases are underlined; the replacement bases are shown in Fig. 12, which is published as supplemental material. Boldfaced letters indicate the sequences from which primers MP723 and MP983 were derived (these two primers were used to amplify a rbcS-m3 3′DNA fragment by PCR for gel shift experiments shown in Fig. 6). (B) Names of the mutation containing constructs and the location of the mutated region in each.