In situ transient expression assays of various
rbcS-m3:GUS reporter genes. Ten-day-old dark-grown maize
seedlings were illuminated for 24 h. Reporter gene DNA was
introduced into segments of leaves of these plants on tungsten
microprojectiles and assays were performed after 24 h of
additional illumination as described in Materials and
Methods. The average number (SEM) of blue spots in MC and BSC
per shot is shown for each mutant chimeric gene. (A)
Control, c; p3′MTnos. mutants, 1, p3′MTnos841; 2, p3′MTnos861; 3,
p3′MTnos900; 4, p3′MTnos925. (B) Control, c; p3′MTnos.
mutant 1, p3′MTnos787. (C) Control, c; p3′MTnos.
mutants, 1, p3′MTnos747; 2, p3′MTnos806; 3, p3′MTnos824.
(D) Control, c; p3′MTnos. mutants 1, p3′MTnos947; 2,
p3′MTnosCTAG; 3, p3′MTnosHOMO.