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. 2001 Feb 27;98(5):2295–2300. doi: 10.1073/pnas.041610098

Figure 6.

Figure 6

TRM1 binds specifically to the HOMO region of the +723 to +983 DNA sequence of rbcS-m3. Gel mobility-shift assays were as described in Materials and Methods. (A) Binding to wild-type DNA. Lane 1, probe +723 to +983 DNA only (Control); lane 2, probe DNA plus TRM1; lanes 3–5, as in lane 2 but with competing wild-type DNA added; lanes 6–8, as in lane 2 but with mutated (M) DNA added; lanes 9–11, as in lane 2 but with nonspecific (NS) DNA added. Lanes 3, 6, and 9 contain 3.4 ng of competitor DNA; lanes 4, 7, and 10 contain 17 ng of competitor DNA; lanes 5, 8, and 11 contain 85 ng of competitor DNA. The wild-type competitor was unlabeled +723 to +983 bp DNA (W). The mutant (M) competitor was +723 to +983 bp DNA with HOMO mutations (see Materials and Methods) in the TRM1 binding site. A 100-bp DNA ladder (NS) (GIBCO/BRL) was used as the nonspecific (NS) competitor DNA. (B) TRM1 binds to wild-type rbcS-m3 +723 to +983 DNA but not to this sequence with mutations in the HOMO region. Lane 1, TRM1 plus wild-type probe DNA, as in A, lane 2 above; lane 2, TRM1 protein plus labeled HOMO mutant DNA.