Skip to main content
. 2001 Feb 27;98(5):2295–2300. doi: 10.1073/pnas.041610098

Figure 7.

Figure 7

TRM1 protein binds to rbcS-m3 5′ sequences. Mobility-shift assays. Probe fragments used were: (A) −555 to −488, (B) −540 to −488, and (C) −540 to −444 rbcS-m3 DNA sequences. Seventy nanograms of purified E .coli TRM1 was used in all lanes except those marked D (which contain labeled DNA only). Lanes P show purified E. coli TRM1 binding to labeled DNA in the absence of competitors. The competition studies were done with increasing concentrations (30, 60, and 120 ng) of unlabeled DNA (SC) or nonspecific (NS) DNA (100-bp DNA ladder from GIBCO/BRL).