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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Cell Physiol. 2011 Mar;226(3):627–637. doi: 10.1002/jcp.22384

Figure 8. Inhibition of ERK and IKKβ blocks the enhanced cytokine levels in GRK2Δmye macrophages.

Figure 8

Peritoneal Mϕ were stimulated or not with LPS along with PD98059 (10 μM) or BMS345541 (5 μM) and the cell culture supernatants collected 24 hours later. Levels of cytokines and chemokines were assessed using Biorad 23-plex assay. Cytokines blocked by PD98059 are shown in (A), cytokines blocked primarily by BMS345541 are shown in (B), and IL-9 is shown in (C). ERKi=PD98059; IKKi=BMS345541; N=5 mice per genotype each *p<0.05; **p<0.01, compared to corresponding GRK2fl/fl. PD98059 or BMS345541 alone did not affect secretion of any of the cytokine/chemokine tested (data not shown).