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. Author manuscript; available in PMC: 2011 Jan 2.
Published in final edited form as: Cell. 2010 Jun 25;141(7):1159–1170. doi: 10.1016/j.cell.2010.05.021

Figure 4. An estimate of the number of active L1Hs elements in an individual (ABC13) genome.

Figure 4

(a) In silico genotyping: The last library in our study, ABC13, was examined in silico (see text) for the presence of insertion fosmids mapping to the location of L1Hs elements found in other individuals. Element 3–17 is used as an example. All blue lines represent insertion fosmids in the genomes of the 8 individuals on the HGSV track (http://hgsv.washington.edu/) of the UCSC genome browser (http://genome.ucsc.edu) (Kent et al., 2002). The ABC7, 8, and 14 libraries were not investigated in this study. (b) PCR validation: The elements identified in silico were genotyped using the scheme shown in Figure 3 to validate the predictions from the HGSV track of the UCSC browser. Element 3–17 is used to illustrate the genotyping. ABC10 and ABC13 are heterozygous with respect to the L1Hs insertion. ABC11 lacks the L1Hs insertion. Table S4 displays genotyping results for all elements in this study.