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. Author manuscript; available in PMC: 2011 Jan 3.
Published in final edited form as: Microvasc Res. 2007 Jan 16;73(2):84–94. doi: 10.1016/j.mvr.2006.10.007

Figure 1. Histological assay to determine direct vascular invasion into fibrin.

Figure 1

A. 20 mg/mL fibrin gels containing 10 ng/mL FGF-2 were placed on the CAM of 10 day old embryos (12 day old embryo is shown in panel A). B. Three days post-placement on the CAM, fluorescent QDs were intravitally injected and the blood vessels were imaged by fluorescence on an M2BIO stereoscope. C. The fibrin and connected CAM were excised and fixed in 1% paraformaldehyde and 3% glutaraldehyde in PBS for 3 hours followed by 3 washes in PBS. Samples were vibratomed perpendicularly to the CAM at 300 μm thickness. The fluorescence of the section shows that blood vessels form up to the fibrin edge but do not directly invade the fibrin.