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. Author manuscript; available in PMC: 2011 Jan 3.
Published in final edited form as: Nat Med. 1995 Oct;1(10):1017–1023. doi: 10.1038/nm1095-1017

Fig. 4.

Fig. 4

Analysis of gene expression in peripheral blood mononuclear cells by the LASN retroviral vector by RT-PCR. RNA from mononuclear cells from patient 1 obtained 6 months (12/93) and 17 months (10/94) after gene therapy were assayed for the frequency of vector-containing cells by semiquantitative RT-PCR as described in the Methods. A standard curve for the frequency of vector-containing cells was produced by diluting cells containing the LASN vector with non-transduced cells. +RT, reversed transcriptase used to copy RNA to DNA; –RT, no reverse transcriptase added.