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. 2001 Feb 13;98(5):2306–2311. doi: 10.1073/pnas.041614598

Figure 2.

Figure 2

Phosphorylation of [3H]Ins(1,4,5)P3 and [3H]Ins(1,3,4,5)P4 by mIPMK. (A) HPLC analysis of assays containing 10 ng of mIPMK incubated with [3H]Ins(1,4,5)P3 for 0, 10, 30, or 120 min. (B) HPLC analysis of assays containing 10 ng of mIPMK incubated with [3H]Ins(1,3,4,5)P4 for 0, 10, 30, or 120 min. The tritium HPLC standards used to identify the products are: (a) Ins(1,4)P2; (b) Ins(1,4,5)P3; (c) Ins(1,3,4,5)P4; (d) Ins(1,3,4,5,6)P5; (e) PP-InsP4; (f) InsP6. These data are representative of three experiments.