. 2010 Sep 10;10(1):M110.000299. doi: 10.1074/mcp.M110.000299

Table II. Identified distinct in vivo phosphorylation sites in isolated mitochondria from human skeletal muscle.

The residue numbering is listed according to the canonical sequences by UniProt/Swiss-Prot.

UniProt accession no.	Gene name	Protein name	Phosphorylation site
Oxidative phosphorylation
O95168	NDUFB4	NADH dehydrogenase 1β subcomplex subunit 4	Ser-26^a
P17568	NDUFB7	NADH dehydrogenase 1β subcomplex subunit 7	Tyr-89
Q7Z4X2 ^a	NDUFB11	NADH dehydrogenase 1β subcomplex subunit 11, variant	Tyr-133,^a Ser-144^a
P08574 ^a	CYC1	Cytochrome c₁, heme protein	Ser-182^a
P00156	CYTB	Cytochrome b	Tyr-75^a
P31930	UQCRC1	Cytochrome b-c₁ complex subunit 1	Ser-107^a
P22695	UQCRC2	Cytochrome b-c₁ complex subunit 2	Thr-369^a
P47985	UQCRFS1	Cytochrome b-c₁ complex subunit Rieske	Ser-99,^a Ser-157^a
P99999	CYCS	Cytochrome c	Thr-29,^a Ser-48^a
P15954	COX7C	Cytochrome c oxidase subunit 7C	Ser-17^a
P25705	ATP5A1	ATP synthase subunit α	Ser-53, Ser-65^a
P06576	ATP5B	ATP synthase subunit β	Thr-213, Ser-529, Ser-415^a
Q5VTU8	ATP5EP2	ATP synthase subunit ϵ-like protein	Thr-29^a
P24539 ^a	ATP5F1	ATP synthase subunit b	Ser-226,^a Thr-229^a
O75947	ATP5H	ATP synthase subunit d	Ser-106^a
P18859 ^a	ATP5J	ATP synthase-coupling factor 6	Ser-64,^a Ser-65^a
P48047	ATP5O	ATP synthase subunit O	Ser-155^a
Q9UII2 ^a	ATPIF1	ATPase inhibitory factor 1 isoform 3	Ser-39^a
Tricarboxylic acid cycle
Q99798	ACO2	Aconitate hydratase	Ser-669^a
O75390	CS	Citrate synthase	Ser-190^a
P10515	DLAT	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex	Ser-475^a
P09622	DLD	Dihydrolipoamide dehydrogenase	Ser-297^a
P48735	IDH2	Isocitrate dehydrogenase (NADP)	Ser-423^a
P40926	MDH2	Malate dehydrogenase, mitochondrial	Ser-51,^a Thr-235, Ser-246, Ser-310^a
A5YVE9	PDHA1	Pyruvate dehydrogenase E1 component subunit α, somatic form	Thr-269, Ser-270, Ser-338, Ser-331
P14618	PKM2	Isoform M1 of pyruvate kinase isozymes M1/M2	Ser-249^a
Q8NBX0 ^a	SCCPDH	Probable saccharopine dehydrogenase	Ser-208^a
Q9P2R7	SUCLA2	Succinyl-CoA ligase (ADP-forming) subunit β	Ser-257, Ser-259^a
Fatty acid transporters and β oxidation
Q92523 ^a	CPT1B	Carnitine O-palmitoyltransferase 1, muscle isoform	Ser-401^a
P43155 ^a	CRAT	Carnitine O-acetyltransferase	Ser-485^a
P42126 ^a	DCI	3,2-trans-Enoyl-CoA isomerase	Ser-285^a
P00505	GOT2	Aspartate aminotransferase, mitochondrial	Ser-360^a
P40939	HADHA	Trifunctional enzyme subunit α	Ser-316,^a Ser-419,^a Ser-669^a
Amino acid degradation
P12694	BCKDHA	2-Oxoisovalerate dehydrogenase subunit α	Ser-337, Ser-347
Q96G95	BCKDK	Branched chain ketoacid dehydrogenase kinase, isoform CRA_a	Ser-31
Import machinery and transporters
P35613	BSG	Isoform 2 of Basigin	Ser-246^a
C9J406	IMMT	Putative uncharacterized protein IMMT	Ser-456^a
Q16891	IMMT	Mitochondrial inner membrane protein	Ser-103,^a Ser-126,^a Ser-307,^a Ser-436,^a Ser-447,^a Ser-507, Tyr-626^a
Q9Y512	SAMM50	Sorting and assembly machinery component 50 homolog	Ser-270
Q02978	SLC25A11	Mitochondrial 2-oxoglutarate/malate carrier protein	Ser-6,^a Tyr-102, Tyr-202, Ser-203^a
O75746 ^a	SLC25A12	Calcium-binding mitochondrial carrier protein Aralar1	Ser-101,^a Ser-662^a
O43772 ^a	SLC25A20	Mitochondrial carnitine/acylcarnitine carrier protein	Ser-205^a
Q59EP7	SLC25A4	Solute carrier family 25 member 4, variant	Ser-200^a
P12235	SLC25A4	ADP/ATP translocase 1	Ser-7, Ser-22, Tyr-191
P05141	SLC25A5	ADP/ATP translocase 2	Thr-84
P12236	SLC25A6	ADP/ATP translocase 3	Tyr-112,^a Ser-119,^a Tyr-195
O60220	TIMM8A	Mitochondrial import inner membrane translocase subunit Tim8 A	Ser-57^a
P21796	VDAC1	Voltage-dependent anion-selective channel protein 1	Ser-13, Ser-43,^a Ser-57, Ser-102,^a Ser-104, Ser-215, Tyr-225,^a Ser-240
P45880	VDAC2	Voltage-dependent anion-selective channel protein 2	Ser-55,^a Ser-57,^a Thr-68,^a Thr-113, Ser-115, Ser-128^a
Q9Y277	VDAC3	Voltage-dependent anion-selective channel protein 3	Thr-4, Thr-6,^a Ser-45,^a Tyr-49, Thr-71^a
Calcium homeostasis
B1AKZ2	CASQ1	Calsequestrin	Ser-191^a
P26678	PLN	Cardiac phospholamban	Ser-16, Thr-17
Apoptosis
O95831	AIFM1	Apoptosis-inducing factor 1	Ser-268, Ser-279,^a Ser-371, Ser-376^a
P17252	PRKCA	Protein kinase C α type	Ser-226
Others
O00116	AGPS	Alkyldihydroxyacetonephosphate synthase, peroxisomal	Ser-65
Q9BUR5 ^a	APOO	Apolipoprotein O	Ser-44^a
Q9GZT3	C14orf156	SRA stem-loop-interacting RNA-binding protein	Ser-102
Q5TGZ0 ^a	C1orf151	UPF0327 protein C1orf151	Ser-4^a
Q9NX63	CHCHD3	Coiled coil-helix-coiled coil-helix domain-containing protein 3	Ser-29,^a Ser-46, Tyr-49, Ser-50
Q9NZ45 ^a	CISD1	CDGSH iron-sulfur domain-containing protein 1 (MitoNEET)	Ser-2,^a Ser-7^a
P17540	CKMT2	Creatine kinase S-type, mitochondrial	Thr-142,^a Thr-143,^a Ser-148,^a Thr-151,^a Tyr-159,^a Ser-163,^a Thr-214,^a Ser-319, Ser-343, Thr-356,^a Tyr-368
O75208	COQ9	Ubiquinone biosynthesis protein COQ9	Thr-178^a
Q9UHQ9	CYB5R1	NADH-cytochrome b₅ reductase 1	Thr-56,^a Tyr-84, Ser-150^a
P00387 ^a	CYB5R3	NADH-cytochrome b₅ reductase 3	Ser-146^a
Q9BWH2	FUNDC2	FUN14 domain-containing protein 2	Ser-151, Ser-167^a
O75323 ^a	GBAS	Protein NipSnap homolog 2	Ser-182^a
P19367	HK1	Hexokinase-1	Ser-337,^a Ser-340^a
P08238	HSP90AB1	Heat-shock protein HSP90-β	Ser-255
P83111 ^a	LACTB	Serine β-lactamase-like protein LACTB	Ser-532^a
P21397	MAOA	Amine oxidase (flavin-containing) A	Tyr-53^a
P27338 ^a	MAOB	Amine oxidase (flavin-containing) B	Ser-374^a
P54296	MYOM2	Myomesin-2	Ser-59,^a Ser-76, Ser-192,^a Thr-600,^a Ser-737,^a Ser-946,^a Ser-951^a
P35232	PHB	Prohibitin	Ser-101,^a Thr-108^a
Q99623 ^a	PHB2	Prohibitin-2	Ser-91,^a Ser-151,^a Thr-155^a
Q9H7Z7 ^a	PTGES2	Prostaglandin E synthase 2	Ser-97^a
P49411	TUFM	Elongation factor Tu	Ser-312^a

^a Novel phosphoproteins/phosphorylation site.