Fig. 2.

Venn diagrams showing the number of proteins identified by Mascot having peptide ion scores of 49 or greater from the one-dimensional gel LC-MS/MS experiment (Strategy 1). A, Total number of proteins identified with or without Na₂CO₃ and KCl washes. B, Number of membrane proteins identified in A having one or more predicted transmembrane segments with or without Na₂CO₃ and KCl washes. The numbers in the square brackets refer to the number of protein clusters. The expression of “w/” means “with” and “w/o” means “without.” Proteins are listed in Supplementary Tables.