Table II. Up-regulated proteins in BMP4-treated cells (signal-to-noise ratio ≥ 10).

Spot no.	Protein name	NCBI accession no.	Theoretical molecular mass	Theoretical pI	Sequence coverage	Matched/searched
			Da		%
1	Crystallin, αB	gi|6753530	20,056.4	6.76	65	13/127
2	Protein-lysine 6-oxidase precursor	gi|126638	46,671.5	8.73	17	5/148
3	Tumor protein, translationally controlled 1	gi|6678437	19,449.6	4.76	53	9/98
4	SH3-binding domain glutamic acid-rich protein-like	gi|9910548	12,803.2	4.99	50	5/169
5	SH3 domain protein 3	gi|22267440	23,767.9	5.46	50	11/100
6	Proteasome subunit β type 2	gi|9910832	22,891.7	6.52	47	11/90
7	Peroxiredoxin 1	gi|6754976	22,162.3	8.26	56	16/102
8	Nucleoside-diphosphate kinase 2	gi|6679078	17,351.9	6.97	59	8/113
9	Cofilin 1, non-muscle	gi|6680924	18,547.7	8.22	41	5/61
10	Peroxiredoxin 6	gi|6671549	24,811	5.98	65	12/26
11	Proteasome (prosome, macropain) subunit, α type 5	gi|7106387	26,394.2	4.74	36	7/63
12	Glutathione S-transferase, Pi 1	gi|10092608	23,594.1	7.68	51	9/90
13	Glyceraldehyde-3-phosphate dehydrogenase	gi|62201487	35,815.2	8.15	48	15/112
14	Predicted: similar to histone H2B F (H2B 291A)	gi|94394734	17,927.5	10.09	52	10/121
15	Similar to phosphoglycerate mutase 1	gi|94369185	28,918.9	6.19	57	12/66
16	FK506-binding protein 1a	gi|6679803	11,915.1	7.88	41	4/107
17	Similar to phosphoglycerate kinase 1	gi|51708092	44,522	8.02	54	20/74
18	Profilin 1	gi|6755040	14,947.5	8.46	73	9/63
19	Pyruvate kinase 3	gi|31981562	57,808	7.18	42	22/124
20	Cystatin B	gi|6681071	11,038.5	6.82	59	7/68
21	Nucleoside-diphosphate kinase 1	gi|37700232	17,196.8	6.84	84	15/113
22	Histidine triad nucleotide-binding protein 1	gi|33468857	13,768.1	6.36	61	7/90
23	Calponin 3, acidic	gi|21312564	36,405.8	5.46	49	14/94
24	Putative β-actin (amino acids 27–375)	gi|49868	39,160.6	5.78	26	16/173
25	RAN-binding protein 1	gi|46397828	23,581.7	5.15	37	6/68
26	S100 calcium-binding protein A4	gi|33859624	11,713.7	5.23	53	8/89
27	Predicted: similar to polyubiquitin isoform 3	gi|94375391	17,399.4	7.93	65	7/67