Table I. Validated Plk1 phosphorylation sites on known spindle proteins.
Known Plk1 substrates and Plk1 phosphorylation site are marked in italic, and conserved sites are marked in bold (species are specified in supplemental Table S3A, column P).
| Proteins | Accession number | Phosphorylation sites |
|---|---|---|
| ARHGEF2 | Q92974 | Ser-737 |
| ASPM | Q8IZT6 | Thr-178, Ser-267, Ser-270, Ser-280, Ser-355, Thr-356, Ser-1825, Ser-3426 |
| AURKB | Q96GD4 | Tyr-239 |
| BLM | P54132 | Ser-26,a Ser-28a |
| Borealin | Q53HL2 | Ser-180 |
| BUB1 | O43683 | Ser-661 |
| CASC5 | Q8NG31 | Ser-1013, Ser-1808 |
| CDC27 | P30260 | Ser-441 |
| CENP-B | P07199 | Ser-306, Ser-307 |
| CENP-C | Q03188 | Ser-96, Ser-104, Ser-110, Thr-516, Ser-250, Thr-734 |
| CENP-E | Q02224 | Ser-611, Ser-612 |
| CENP-F | P49454 | Ser-242, Ser-838, Ser-1248, Ser-1324, Ser-1750, Ser-1988, Ser-2512, Ser-2513 |
| CENP-I | Q92674 | Ser-709 |
| CENP-J (CPAP) | Q9HC77 | Ser-556a |
| CENP-L | Q8N0S6 | Ser-53 |
| CENP-N | Q96H22 | Ser-226, Ser-235, Ser-282 |
| CENP-T | Q96BT3 | Ser-45 |
| CENPU (PBIP1) | Q71F23 | Ser-194 |
| C14Orf106 | Q6P0N0 | Ser-135, Ser-192 |
| Cep97 | Q8IW35 | Ser-308 |
| Cep170 | Q5SW79 | Ser-880a |
| Cep192 | Q8TEP8 | Ser-1502 |
| CDK5 (Cep215) | Q96SN8 | Ser-613, Ser-1102 |
| Cep350 | Q5VT06 | Ser-2689 |
| DGL7 (HURP) | Q86T11 | Ser-777 |
| ERCC6L (PICH) | Q2NKX8 | Ser-774, Ser-790 |
| INCENP | Q9NQS7 | Ser-72, Ser-330 |
| KIF18A | Q8NI77 | Ser-681a |
| KIF20A | O95235 | Tyr-558, Ser-635 |
| KIF23 | Q02241 | Ser-867,aThr-897, Ser-889 |
| KIFC1 (HSET) | Q9BW19 | Ser-33 |
| KIF4A | O95239 | Ser-394, Ser-815, |
| LB1 | Q8WWK9 | Ser-533 |
| MAD1 | Q9Y6D9 | Ser-8,a Ser-490a |
| NCAPD3 | P42695 | Thr-430, Ser-508 |
| NCAPG2 | Q86XI2 | Ser-30, Thr-1114 |
| NCAPH | Q15003 | Thr-98 |
| NPM | P06748 | Ser-198 |
| NuMA | Q14980 | Thr-1818 |
| NUP88 | Q99567 | Ser-540 |
| NUP93 | Q8N1F7 | Ser-72 |
| NUP98 | P52948 | Ser-591 |
| NUP107 | P57740 | Ser-4, Ser-57 |
| NUP153 | P49790 | Ser-343 |
| PCM1 | Q15154 | Ser-110 |
| PCNT | O95613 | Ser-813, Ser-815, Thr-1688, Thr-1690, Ser-2594, Thr-3325, Ser-3326 |
| PRC1 | O43663 | Ser-554, Ser-592 |
| RACGAP1 | Q9H0H5 | Ser-164, Ser-170, Ser-214 |
| SGOL2 | O562F6 | Ser-436, Ser-1151a |
| SON | P18583 | Ser-154 |
| SPAG5 (ASTRIN) | Q96R06 | Ser-401 |
| Sperm-specific antigen 2 | P28290 | Ser-737, Ser-739 |
| TOP2A | P11388 | Ser-282 |
| TPR | P12270 | Ser-1185 |
| TPX2 | Q9ULW0 | Ser-356, Thr-361, Ser-654 |
| TUBA1C | Q9BQE3 | Ser-41, Ser-48 |
| TUBGCP5 | Q96RT8 | Ser-182 |
| Vimentin | P08670 | Ser-83, Ser-409, Thr-458, Ser-459 |
| WD62 | Q9UFV9 | Ser-974 |
| ZWINT | O95229 | Ser-84a |
a Sites that were identified with high confidence in earlier experiments (analyzed with MSQuant) but only reached a final MASCOT score <12 in this study (and hence were not included in supplemental Table S3A).