Figure 2.

Active vitamin D compounds but not cinacalcet normalize an unmineralized growth plate and unmineralized bone. Representative von Kossa stains of (A) the growth plates and (B) the metaphyses are shown: of the tibiae of wild-type mice; of 1α(OH)ase null mice [1α(OH)ase^−/−] on a high-calcium intake (h) treated with vehicle, 1,25(OH)₂D₃, doxercalciferol, and cinacalcet; and of 1α(OH)ase null mice [1α(OH)ase^−/−] on a rescue diet (r) treated with vehicle, and cinacalcet. Black staining denotes mineralization; purple staining depicts the collagen in the growth plates. The white arrows on the black mineralized trabeculae denote the absence or presence of unmineralized bone, which stains light blue. Red arrows denote osteoclasts lining the bone of cinacalcet-treated null mice on a rescue diet. Magnification: ×100 in A; ×200 in B.