Figure 1.

miR-192 and miR-215 target sequences localize within the 3′UTR common to both WNK1 isoforms. miR-192 and miR-215 target sequences span from position 542 to 562 relative to the stop codon, in WNK1 3′UTR. The seed sequence of miR-192 and miR-215 completely matches with the sequence extending from 554 to 562 within the 3′UTR of WNK1 (sequence highlighted in white). This sequence is conserved among species. The target sequence is located upstream of WNK1 first polyadenylation signal (pA1), which is common to both isoforms as indicated in the schematic depiction of L-WNK1 and KS-WNK1.