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. Author manuscript; available in PMC: 2011 Dec 16.
Published in final edited form as: Cell Host Microbe. 2010 Dec 16;8(6):484–495. doi: 10.1016/j.chom.2010.11.005

Figure 3. ROP18 blocks Irgb6 loading.

Figure 3

(A) Immunofluorescence localization of Irgb6 on the parasite-containing vacuole in infected Gr1+ monocytes examined at 30 min post infection. Irgb6 localized with rabbit polyclonal Ab (Alexa Fluor 488, green); GRA5 localized with mAb Tg 17-113(Alexa Fluor 594, red); and nuclei stained with DAPI (blue). Scale = 5 μm.

(B) Quantification of Irgb6 localization to the PV in Gr1+ monocytes. Mean ± SEM, n=3 experiments (*P < 0.005, Student's t test).

(C) CryoimmunoEM localizing Irgb6 to the parasite-containing vacuole membrane (arrowheads) in macrophages. Scale = 0.5 μm.