Table 1.

Primer sequences for amplifying soluble PBPs 5 and 6, and their mosaics

Primer	Primer sequence
P1	5′-CTC TCT CTC ATA TGG CGG AAC AAA CCG TTG AAG CGC CGA GC-3′
P2	5′-CTC TCT CTA AGC TTT CAT CCG CCC TCT TCC ACA TTT TCC ATC AC -3′
P3	5′-CTC TCT CTC ATA TGG ATG ACC TGA ATA TCA AAA CTA T -3′
P4	5′-GCA TGC AAG CTT TCA ATC GAT CAC CGG ATC GCC GAA GTT ACC TTC CGG GAT TTC -3′
P5	5′-GCA TGC AAG CTT TCA ATC CCA CAC CCG ACC AAA GAA TCC GCC-3′

Underlined sequences are complementary to the 5′ or 3′ end of the gene fragment in each construct. Boldfacing designates enzyme recognition sites: AAGCTT, Hind III; CATATG, Nde I.