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Thermal Denaturation of WT and D187N S2 as well as WT, D187N, and D187Y S2loop gelsolin monitored by CD at 213 nm. WT data are shown in black circles, D187N data in red triangles, and D187Y data in blue squares. Solid lines represent the best fits by a nonlinear least squares analysis to Eq. 2. (A) Gelsolin S2 WT and D187N (10% glycerol). (B) WT, D187N, and D187Y gelsolin S2loop.
