Figure 5.

Accumulation of AtBI-1 mRNA in Arabidopsis Cells under Oxidative Stress or in NahG Transgenic Plants.

(A) Arabidopsis suspension cells (3 days old) were treated with H₂O₂ (1 and 3 mM) or SA (500 and 750 μM) for 24 h under continuous shaking. Equal amounts of total RNAs (10 μg) were electrophoresed on a 1.2% formaldehyde agarose gel and transferred to a membrane. An α-³²P-dCTP–labeled 3′ untranslated region of AtBI-1 cDNA was used as a probe.

(B) Analysis of AtBI-1 mRNA accumulation in NahG transgenic plants. Total RNAs (20 μg) from 2-week-old wild-type (WT) and NahG transgenic plants were electrophoresed and hybridized.

The middle panels in (A) and (B) show gels stained with ethidium bromide for rRNA. The bottom panels show relative levels of AtBI-1 expression in Arabidopsis suspension cells treated with H₂O₂ and SA (A) and NahG transgenic plants (B). Quantitative measurements of RNA gel blots were performed using the BAS1500 imaging plate scanner.