Figure 1.

Characterization of the γ-Tubulin Genes of Basal Land Plants.

(A) Deduced phylogeny tree generated by the unweighted pair group method with arithmetic mean algorithm. The γ-tubulins used to generate the tree are listed in (B).

(B) Amino acid sequence comparison of the C. japonicum and other plant γ-tubulins. The deduced amino acid sequence of the C. japonicum γ-tubulin is shown in the top row. Amino acid sequences of plant γ-tubulins were aligned, and amino acids identical to those of the C. japonicum γ-tubulin are indicated by dashes. Dots indicate gaps introduced for the alignment. The inferred recognition epitope of the G9 antibody is underlined.

H. sap, Homo sapiens; X. lae, Xenopus laevis; A. tha (At), Arabidopsis thaliana; N. tab (Nt), Nicotiana tabacum; Z. may (Zm), Zea mays; C. jap (Cj), Conocephalum japonicum; P. pat (Pp), Physcomitrella patens; A. phy (Ap), Anemia phyllitidis; C. rei (Cr), Chlamydomonas reinhardtii; S. pom (Sp), Schizosaccharomyces pombe; A. nid, Aspergillus nidulans; S. cer, Saccharomyces cerevisiae.