Figure 4.

Changes in the Subcellular Localization of γ-Tubulin Homologs during Plastid Division and Partitioning in Monoplastidic Meiosis of D. hirsuta (Premeiotic Interphase to Prophase I).

(A) and (B) A premeiotic interphase cell with a single plastid (asterisk in [B]). Note that dots of γ-tubulin homologs are distributed randomly in the cytoplasm (B) with reticular arrays of microtubules (A).

(C) and (D) An early prophase cell with two daughter plastids (asterisks in [D]). Note the γ-tubulin homologs localized around the two plastids.

(E) to (H) A mid-prophase cell with four daughter plastids (asterisks in [F]). Note that the localization of γ-tubulin homologs around the four daughter plastids becomes more prominent. (G) and (H) show single optical sections of (E) and (F), respectively.

(I) and (J) A mid-prophase cell with four daughter plastids (asterisks in [I]). The primary antibody (G9) is omitted (a similar stage is shown in [E]). Crosstalk and nonspecific signal of secondary antibody were not observed.

Microtubules (green signal in [A], [C], [E], [G], and [I]), DNA (blue signal in [A], [C], [E], [G], and [I]), and γ-tubulin homologs (red signal in [B], [D], [F], [H], and [J]) were stained using anti-plant-tubulin, DAPI, and G9 anti-γ-tubulin, respectively. (A) and (B), (C) and (D), (E) and (F), (G) and (H), and (I) and (J) show the same cells with different color combinations. Bar = 10 μm.