Figure 5.

MeJA-Induced Gene Expression in Wild-Type and jai1-1 Plants.

Three-week-old Micro-Tom and jai1-1 plants were exposed to MeJA vapor for various lengths of time (hours) in an enclosed box. Leaves from similarly treated plants of the same genotype were pooled for RNA extraction. RNA isolated from untreated plants (0-h time point) also was analyzed as a control. RNA gel blots were hybridized to cDNA probes representing four late genes (PI-I, PI-II, CATHEPSIN D INHIBITOR [CDI], and THREONINE DEAMINASE [TD]) and four early genes (LIPOXYGENASE D [LoxD], ALLENE OXIDE SYNTHASE2 [AOS2], OPDA REDUCTASE3 [OPR3], and PROSYSTEMIN [PSYS]). Blots also were hybridized to the LeCoi1 cDNA and, as a loading control, to an eIF4A probe.