Figure 6.

HMGB1 blockade inhibits mRNA upregulation of downstream proinflammatory cytokines and chemokines in kidney IRI. Real-time PCR demonstrated that mRNA expression of IL-6 (A), TNFα (B), and MCP-1 (C) in the kidney was significantly reduced in anti-HMGB1 Ab-treated (black bars) mice compared with Ctrl Ig-treated mice (gray bars) on days 1 and 5 after reperfusion. The results have been normalized by expressing the number of transcript copies as a ratio to GAPDH. The data shown are the means ± SD. n = 5 to 9 per group.