Figure 7.

Administration of recombinant HMGB1 worsens kidney IRI. (A and B) Compared with sham-operated control, IR caused significant increases in the level of serum creatinine (A) and the tubular damage score (B) in PBS-treated mice at day 1 post-IR. Kidney injury was exacerbated in mice treated with rHMGB1 (n = 5) with higher serum creatinine (A) and more severe tubular damage (B) than PBS-treated mice (n = 7). (C and D) Significant infiltration of neutrophils and macrophages in the kidney was evident in both PBS- and rHMGB1-treated mice at day 1 post-IR compared with sham-operated mice with a trend toward greater numbers of both cell types in rHMGB1-treated mice. (E through G) rHMGB1-treated WT mice showed a significant increase in IL-6, TNFα, and MCP1 expression compared with PBS-treated mice at 24 hours post-IR.