Figure 1.
Anti-dsDNA antibodies bind to HMC. (A) Flow cytometric histograms of HMC that have been incubated with control human IgG (left panel) or human polyclonal anti-dsDNA antibodies (middle panel). Preincubation of anti-dsDNA antibodies with exogenous DNA (1 μg/ml) before their addition to HMC inhibited their binding to HMC (right panel). (B) Binding of SFM, control human IgG, or anti-dsDNA antibodies to HMC in cellular ELISA before and after removal of cell surface proteins with limited trypsinization (10 μg/ml). The amount of IgG bound to HMC was expressed as μg of bound IgG/μg of cellular protein.21 The horizontal line represents the mean μg of bound IgG/μg of cellular protein for each group.